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Proceedings Paper

High speed and large effective range autofocusing based on numerical wavefront propagation
Author(s): Jing Xu; Zhilong Jiang; Yan Kong; Shumei Gao; Cheng Liu; Shouyu Wang
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Paper Abstract

Autofocusing is widely used in microscopy since it provides sample details with high resolution and contrast. However, massive image recording along optical axis is often indispensable in classical autofocusing tactics, obviously decreasing autofocusing speed. To increase processing efficiency, we propose numerical wavefront propagation based autofocusing with high speed and large effective range. Firstly, quantitative sample phase is retrieved from multi-focal images according to transport of intensity equation with Gerchberg-Saxton algorithm. Then, various intensities along optical axis are numerically computed via wavefront propagation, thus focal position can be determined according to in-focus criterion. Finally, in-focus image is recorded via sample stage shifting. Since the proposed autofocusing approach can be easily integrated into commercial microscopes, additionally, it considerably reduces image captures as well as time consuming, we believe it can be adopted for rapid autofocusing in microscope.

Paper Details

Date Published: 23 October 2018
PDF: 8 pages
Proc. SPIE 10820, Optics in Health Care and Biomedical Optics VIII, 108201N (23 October 2018); doi: 10.1117/12.2500586
Show Author Affiliations
Jing Xu, Jiangnan Univ. (China)
Zhilong Jiang, Jiangnan Univ. (China)
Yan Kong, Jiangnan Univ. (China)
Shumei Gao, Jiangnan Univ. (China)
Cheng Liu, Jiangnan Univ. (China)
Shouyu Wang, Jiangnan Univ. (China)

Published in SPIE Proceedings Vol. 10820:
Optics in Health Care and Biomedical Optics VIII
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang, Editor(s)

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