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Proceedings Paper • new

Digital field-of-view correction for dual-view transport-of-intensity equation-based quantitative microscopy
Author(s): Mingyuan Zhang; Qingtao Gong; Qi Wei; Jing Xu; Yan Kong; Zhilong Jiang; Cheng Liu; Shouyu Wang
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Paper Abstract

As an ideal way for quantitative live cell imaging, dual view transport of intensity equation (TIE) method can provide both real time imaging, multi-mode observations, simple setup and large field of view (FoV). However, the image recorder installation error reduces the accuracy in both amplitude and phase retrievals, because of the inevitable FoV mismatch between the captured under- and over-focus intensities. In order to obtain higher accuracy amplitude and phase retrievals, the phase correlation based digital FoV correction is introduced into our method, rotation, scale and translation between the under- and over-focus images are compensated by the phase correlation based digital FoV correction. Measurements are implemented using standard sample detection and quantitative live cell imaging, proving that the proposed method can improve the accurate of the amplitude and phase computations.

Paper Details

Date Published: 23 October 2018
PDF: 7 pages
Proc. SPIE 10820, Optics in Health Care and Biomedical Optics VIII, 108201M (23 October 2018); doi: 10.1117/12.2500585
Show Author Affiliations
Mingyuan Zhang, Jiangnan Univ. (China)
Qingtao Gong, Jiangnan Univ. (China)
Qi Wei, Jiangnan Univ. (China)
Jing Xu, Jiangnan Univ. (China)
Yan Kong, Jiangnan Univ. (China)
Zhilong Jiang, Jiangnan Univ. (China)
Cheng Liu, Jiangnan Univ. (China)
Shouyu Wang, Jiangnan Univ. (China)


Published in SPIE Proceedings Vol. 10820:
Optics in Health Care and Biomedical Optics VIII
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang, Editor(s)

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