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Proceedings Paper

Mechanism of melanosomes action in antioxidant reactions following optic irradiation
Author(s): Victoria Alkseyevna Lapina; Alexander E. Dontsov; Natalia L. Sakina; Michail A. Ostrovsky
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Paper Abstract

Hyphema is often associated with traumatic eye injuries. Its blood is subjected to considerable changes in aqueous humor and causes toxic effect on eye tissues. The metabolism disturbances in eye tissues unfavorably affecting their cell structures play a significant role in the development of pathologic conditions in cases of intraocular hemorrhages. This is to be attributed to the correlation of lipid peroxidation (LP) processes and antioxidant system (AOS) activity of both the cellular membrane and its environment necessary for maintaining cell homeostasis as a whole. The aim of the present work is an experimental study of LP processes of aqueous humor (AH) in total traumatic hyphema (TTH) after magnet-laser stimulation different doses (3 and 6 minutes). TTH and aqueous humor collection were made on the eyes on rabbit (Schinchilla) according to Krasnov A.M. under the local anaesthesia (dicaine 0.25%). The study of biochemical aqueous indices was carried out on healthy eyes and after development of hyphema on day 1, 3, 5, 7, 10, 14, 30, and 45. The animals were divided into three groups: in the first group the biochemical indices were defined in the aqueous humor in TTH; in the second and the third groups the similar investigation was carried out after magnet- laser radiation with 3 and 6 minutes exposition. The malonic dialdehyde (MDA) was defined according to Ishihara, Shiff's bases -- and their concentration was determined on the total amount of lipids (reagent kit of 'Lachema'). He-Ne laser (with power density of 0.05 mVt) was used, streaming impulsed magnet field voltage being 10 mTl. The magnet head of a magnet radiator was fixed 0.5 cm from the eye; laser beam being sent to the central hole of the magnet head. The duration of exposure was 3 minutes in the second group and 6 minutes in the third group, daily for 10 days. Significant increase of LP product concentration level with two clear ascending peaks was seen on the third and tenth days in the first group. The maximal increase of these indices was noted on the third day, that coincides with hemolysis of outpouring blood and release of powerful LP processes initiators into aqueous humor -- that is haemoglobin and Fe++2. The MDA and Shiff's bases concentration levels increased equally during this period with reference to their initial data, whereas on the tenth day the level of Shiff's bases concentration became higher than the MDA amount level. The normalized values of the indices studied were obtained on the 30 - 45th days. The use of magnet-laser radiation caused the significant fall in MDA concentration and Shiff's bases in aqueous humor in TTH in the second group (1.8 and 1.7 correspondingly). On the 7th - 10th days already the values of indices studied were reliably similar to norm (P greater than 0.05). In the third group the levels of LP product concentration fell in the first phase of hyphema resolution. It had been determined that the MDA values were lower than the values of indices of the first group on the third - fifth days (1.5 and 1.7 correspondingly). The values of Shiff's bases on the first day were 1.4 lower than the indices of the first group and were reliably similar to indices of the first group on the third - seventh days (P greater than 0.05).

Paper Details

Date Published: 17 May 1996
PDF: 6 pages
Proc. SPIE 2673, Ophthalmic Technologies VI, (17 May 1996); doi: 10.1117/12.240067
Show Author Affiliations
Victoria Alkseyevna Lapina, Institute of Physics (Belarus)
Alexander E. Dontsov, Institute of Biological Physics (Russia)
Natalia L. Sakina, Institute of Biological Physics (Russia)
Michail A. Ostrovsky, Institute of Biological Physics (Russia)


Published in SPIE Proceedings Vol. 2673:
Ophthalmic Technologies VI
Jean-Marie A. Parel; Karen Margaret Joos; Pascal O. Rol, Editor(s)

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