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Proceedings Paper

In-vivo immunofluorescence confocal microscopy of herpes simplex virus type 1 keratitis
Author(s): Stephen C. Kaufman M.D.; Jeffery A. Laird; Roger W. Beuerman
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Paper Abstract

The white-light confocal microscope offers an in vivo, cellular-level resolution view of the cornea. This instrument has proven to be a valuable research and diagnostic tool for the study of infectious keratitis. In this study, we investigate the direct visualization of herpes simplex virus type 1 (HSV-1)-infected corneal epithelium, with in vivo confocal microscopy, using HSV-1 immunofluorescent antibodies. New Zealand white rabbits were infected with McKrae strain of HSV-1 in one eye; the other eye of each rabbit was used as an uninfected control. Four days later, the rabbits were anesthetized and a cellulose sponge was applied to each cornea, and a drop of direct HSV fluorescein-tagged antibody was placed on each sponge every 3 to 5 minutes for 1 hour. Fluorescence confocal microscopy was then performed. The HSV-infected corneas showed broad regions of hyperfluorescent epithelial cells. The uninfected corneas revealed no background fluorescence. Thus, using the confocal microscope with a fluorescent cube, we were able to visualize HSV-infected corneal epithelial cells tagged with a direct fluorescent antibody. This process may prove to be a useful clinical tool for the in vivo diagnosis of HSV keratitis.

Paper Details

Date Published: 17 May 1996
PDF: 4 pages
Proc. SPIE 2673, Ophthalmic Technologies VI, (17 May 1996); doi: 10.1117/12.240044
Show Author Affiliations
Stephen C. Kaufman M.D., Louisiana State Univ. Eye Ctr. (United States)
Jeffery A. Laird, Louisiana State Univ. Eye Ctr. (United States)
Roger W. Beuerman, Louisiana State Univ. Eye Ctr. (United States)

Published in SPIE Proceedings Vol. 2673:
Ophthalmic Technologies VI
Jean-Marie A. Parel; Karen Margaret Joos M.D.; Pascal O. Rol, Editor(s)

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