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Proceedings Paper

Corneal collagen denaturation in laser thermokeratoplasty
Author(s): Ralf Brinkmann; Juergen Kampmeier; Ulf Grotehusmann; Alfred Vogel; Norbert Koop; Mary Asiyo-Vogel; Reginald Birngruber
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Paper Abstract

In laserthermokeratoplasty (LTK) thermal denaturation and shrinkage of corneal collagen is used to correct hyperopia and astigmatism. In order to optimize dosimetry, the temperature at which maximal shrinkage of collagen fibrils occurs is of major interest. Since the exposure time in clinical LTK-treatment is limited to a few seconds, the kinetics of collagen denaturation as a rate process has to be considered, thus the time of exposure is of critical importance for threshold and shrinkage temperatures. We investigated the time-temperature correlation for corneal collagen denaturation within different time domains by turbidimetry of scattered HeNe laser probe light using a temperature controlled water bath and pulsed IR laser irradiation. In the temperature range of 60 degree(s)C to 95 degree(s)C we found an exponential relation between the denaturation time and temperature. For the typical LTK-treatment time of 2 s, a temperature of 95 degree(s)C is needed to induce thermal damage. Use of pulsed Holmium laser radiation gave significant scattering of HeNe laser probe light at calculated temperatures of around 100 degree(s)DC. Rate parameters according to the formalism of Arrhenius were fitted to these results. Force measurements showed the simultaneous onset of light scattering and collagen shrinkage.

Paper Details

Date Published: 7 May 1996
PDF: 8 pages
Proc. SPIE 2681, Laser-Tissue Interaction VII, (7 May 1996); doi: 10.1117/12.239611
Show Author Affiliations
Ralf Brinkmann, Medizinisches Laserzentrum Luebeck GmbH (Germany)
Juergen Kampmeier, Medizinisches Laserzentrum Luebeck GmbH (Germany)
Ulf Grotehusmann, Medizinisches Laserzentrum Luebeck GmbH (Germany)
Alfred Vogel, Medizinisches Laserzentrum Luebeck GmbH (Germany)
Norbert Koop, Medizinisches Laserzentrum Luebeck GmbH (Germany)
Mary Asiyo-Vogel, Medizinisches Laserzentrum Luebeck GmbH (Germany)
Reginald Birngruber, Medizinisches Laserzentrum Luebeck GmbH (Germany)


Published in SPIE Proceedings Vol. 2681:
Laser-Tissue Interaction VII
Steven L. Jacques, Editor(s)

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