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Proceedings Paper

Photochemical cross-linking of type I collagen with hydrophobic and hydrophilic 1,8-naphthalimide dyes
Author(s): Millard M. Judy; Li Chen; L. Fuh; Hany R. Nosir; Robert W. Jackson; James Lester Matthews; David E. Lewis; Ronald E. Utecht; Dongwu Yuan
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Paper Abstract

This study extends our previous studies of the photochemical cross-linking of collagen and bonding of collagenous sheets of dura mater using the hydrophobic 1,8-naphthalimide N,N'- bis-(2-({hexylamino)-5-bromo-1H-benz [de]isoquinolin-1,3(2H)-dion-6- yl]amino}ethyl)hexanediamide. We have obtained hydrophilic forms of the bifunctional molecule introducing a spacer and ligands containing alternating carbon-oxygen bonds (polyethers) wherein the oxygen moieties form hydrogen bonds. Additional hydrophilicity is attained by incorporating of an amino group (positive charge) at the end of each ligand. Ongoing studies with these forms of the bifunctional 1,8 naphthalimides have demonstrated welding of meniscal cartilage, articular cartilage, and cornea. These results suggest that the hydrophilic form of the dyes is able to penetrate readily the anionically charged proteoglycan matrix of these tissues and cross-link collagen molecules and possibly the protein cores of the proteoglycans. Gel electrophoretic studies have been performed to assess the photochemical cross-linking of these connective tissue proteins with these new forms of the paththalimide dyes.

Paper Details

Date Published: 7 May 1996
PDF: 3 pages
Proc. SPIE 2681, Laser-Tissue Interaction VII, (7 May 1996); doi: 10.1117/12.239610
Show Author Affiliations
Millard M. Judy, Baylor Research Institute and MicroBioMed Corp. (United States)
Li Chen, Baylor Research Institute (United States)
L. Fuh, Baylor Research Institute (United States)
Hany R. Nosir, Baylor Univ. Medical Ctr. (United States)
Robert W. Jackson, Baylor Univ. Medical Ctr. (United States)
James Lester Matthews, Baylor Research Institute and MicroBioMed Corp. (United States)
David E. Lewis, MicroBioMed Corp. and South Dakota State Univ. (United States)
Ronald E. Utecht, MicroBioMed Corp. and South Dakota State Univ. (United States)
Dongwu Yuan, MicroBioMed Corp. and South Dakota State Univ. (United States)


Published in SPIE Proceedings Vol. 2681:
Laser-Tissue Interaction VII
Steven L. Jacques, Editor(s)

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