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Proceedings Paper

Internal absorption coefficient and threshold for pulsed laser disruption of melanosomes isolated from retinal pigment epithelium
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Paper Abstract

This study determine the threshold radiant exposure from a 10-ns pulsed laser (532 nm) which caused bovine melanosomes to present various observable endpoints of disruption. The endpoints tested were (1) a visible region of clearing in a uniform field of melanosomes under a glass cover slip, (2) an audible sound, and (3) the increase in melanin photoreactivity due to photodisruption of melanosomes. The thresholds were tested for different starting temperatures by pre-equilibrating the melanosomes in aqueous solution at various temperatures. Lower temperatures required larger exposures to attain a given endpoint. The data suggest that bovine RPE melanosomes are about 4-fold denser in melanin content than cutaneous melanosomes.

Paper Details

Date Published: 7 May 1996
PDF: 10 pages
Proc. SPIE 2681, Laser-Tissue Interaction VII, (7 May 1996); doi: 10.1117/12.239608
Show Author Affiliations
Steven L. Jacques, Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Randolph D. Glickman, Univ. of Texas Health Science Ctr./San Antonio (United States)
Jon A. Schwartz, Univ. of Texas M.D. Anderson Cancer Ctr. (United States)


Published in SPIE Proceedings Vol. 2681:
Laser-Tissue Interaction VII
Steven L. Jacques, Editor(s)

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