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Proceedings Paper

Two-photon excitation laser scanning microscopy: applications in neuroscience
Author(s): Winfried Denk
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Paper Abstract

High resolution fluorescence imaging in intact tissues faces special challenges posed by scattering of excitation and fluorescence light and the need to avoid photodynamic damage. Significant improvements over conventional widefield and confocal imaging are provided when two-photon excitation is used. Applications to the functional imaging of the calcium dynamics in synaptic spines, small invertebrate neurites, and auditory hair cells are shown. Two-photon absorption induced photolysis can also be used for scanning photochemical microscopy and for high resolution measurements of diffusional coupling between cellular compartments.

Paper Details

Date Published: 10 May 1996
PDF: 3 pages
Proc. SPIE 2678, Optical Diagnostics of Living Cells and Biofluids, (10 May 1996); doi: 10.1117/12.239549
Show Author Affiliations
Winfried Denk, AT&T Bell Labs. (United States)


Published in SPIE Proceedings Vol. 2678:
Optical Diagnostics of Living Cells and Biofluids
Daniel L. Farkas; Robert C. Leif; Alexander V. Priezzhev; Toshimitsu Asakura; Bruce J. Tromberg, Editor(s)

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