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Proceedings Paper

Reaction of lymphoid organs to laser radiation with different pulsation rates
Author(s): Igor K. Kapinosov; Irina O. Bugaeva; George R. Kolokolov; Helen J. Provozina
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Paper Abstract

Experimental studies were performed on 220 male rats of Wistar line to reveal optimal parameters of laser radiation causing positive changes in biotissues and to select methods of laser therapy. Irradiation of the ventral abdominal wall performed by arsenide-gallium injector (710 - 890 nm, exposure - 128 sec) in pulse rate: 3000 Hz, 1500 Hz, 80 Hz. Content of lymphoblasts, medium and small lymphocytes, plasmocytes, T-lymphocytes and T-helpers as well as the activity of chromatin and lysosomal enzymes were determined in the dynamics of thymus, spleen and lymph nodes. During irradiation with the rate of 3000 Hz prevailing inhibiting influence on the immumocytopoesis and functional activity of lymphocytes in all organs studied was state, the effect being manifested by the decrease in the number of all forms of lymphocytes particular on the 3rd-5th-7th day followed by normalization on the 15th-21st-30th day. Irradiation with the rate of 1500 Hz produced stimulating effect on the immune organs accompanied by reliable excess of control indices of lymphocyte content particularly of poorly differentiated forms (blasts and medium ones), as well as by the increase of the number of plasmocytes, T-lymphocytes, T-helpers with maximum manifestation on the 7th day. On the 15th day there is a decrease, and on the 21st-30th day--there is normalization. Irradiation with the rate of 80 Hz produced the smallest but most marked effect, particularly on the number of lymphoblasts. Peculiarities in kinetics of cellular elements studied were revealed in different lymphoid organs and in different functional zones of these organs.

Paper Details

Date Published: 10 May 1996
PDF: 4 pages
Proc. SPIE 2678, Optical Diagnostics of Living Cells and Biofluids, (10 May 1996); doi: 10.1117/12.239543
Show Author Affiliations
Igor K. Kapinosov, Saratov State Medical Univ. (Russia)
Irina O. Bugaeva, Saratov State Medical Univ. (Russia)
George R. Kolokolov, Saratov State Medical Univ. (Russia)
Helen J. Provozina, Saratov State Medical Univ. (Russia)

Published in SPIE Proceedings Vol. 2678:
Optical Diagnostics of Living Cells and Biofluids
Daniel L. Farkas; Robert C. Leif; Alexander V. Priezzhev; Toshimitsu Asakura; Bruce J. Tromberg, Editor(s)

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