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Proceedings Paper

Spectral investigation of somatotropin for different pH values
Author(s): Virginia Otero de Joshi; Herminia Gil; Silvia Contreras; Narahari V. Joshi; Luis Hernandez
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Paper Abstract

Spectral investigations of absorbance in deep ultra-violet region (from 200 nm to 350 nm) of (STM) was carried out for different pH values. On the high energy side the peak is located at 195 nm which is generally attributed to peptide bonds. This peak, as expected, does not show any shift with pH value (4.3 to 10.8). A rather broad peak is spread in the region from 200 nm to 240 nm which could be the superposition of the peaks corresponding to the absorption due to (alpha) helix and (beta) structure. This peak shows a red shift as pH value increases. The same hormone was glycated by a conventional method and the process was estimated with the absorption spectra. The results are discussed in the light of nonenzymatic glycation. It was found that glycation mucus somatotropin resistant towards the denaturation process.

Paper Details

Date Published: 24 April 1996
PDF: 6 pages
Proc. SPIE 2676, Biomedical Sensing, Imaging, and Tracking Technologies I, (24 April 1996); doi: 10.1117/12.238807
Show Author Affiliations
Virginia Otero de Joshi, Univ. de Los Andes (Venezuela)
Herminia Gil, Univ. de Los Andes (Venezuela)
Silvia Contreras, Univ. de Los Andes (Venezuela)
Narahari V. Joshi, Univ. de Los Andes (Venezuela)
Luis Hernandez, Univ. de Los Andes (Venezuela)


Published in SPIE Proceedings Vol. 2676:
Biomedical Sensing, Imaging, and Tracking Technologies I
Robert A. Lieberman; Halina Podbielska; Tuan Vo-Dinh, Editor(s)

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