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Proceedings Paper

Development of a genosensor for Mycobacterium tuberculosis
Author(s): Narayana R. Isola; Jean Pierre Alarie; Guy D. Griffin; Tuan Vo-Dinh
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Paper Abstract

This work involves the development of a fiberoptic genosensor for the detection of Mycobacterium tuberculosis. A rapid and sensitive non-radioactive method for the detection of Mycobacterium is described. This method involves polymerase chain reaction (PCR) amplification of the target DNA utilizing gene specific primers labelled with fluorescein at the 5' end and hybridization of the amplified product to a species specific oligonucleotide probe sequence covalently linked to the end of a fiber. The fluorescent hybridization products are detected by laser induced fluorescence using an argon laser (488 nm).

Paper Details

Date Published: 24 April 1996
PDF: 13 pages
Proc. SPIE 2676, Biomedical Sensing, Imaging, and Tracking Technologies I, (24 April 1996); doi: 10.1117/12.238803
Show Author Affiliations
Narayana R. Isola, Oak Ridge National Lab. (United States)
Jean Pierre Alarie, Oak Ridge National Lab. (United States)
Guy D. Griffin, Oak Ridge National Lab. (United States)
Tuan Vo-Dinh, Oak Ridge National Lab. (United States)


Published in SPIE Proceedings Vol. 2676:
Biomedical Sensing, Imaging, and Tracking Technologies I
Robert A. Lieberman; Halina Podbielska; Tuan Vo-Dinh, Editor(s)

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