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Proceedings Paper

Molecular assembly of multilayer enzyme on fiber optic surface: toward the development of chemiluminescence-based fiber optic biosensors
Author(s): Zhongping Chen; Harry Hong Gao; Jayant Kumar; Kenneth A. Marx; Sukant K. Tripathy; David L. Kaplan
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Paper Abstract

We report here on a technique to immobilize a multilayer enzyme assembly on an optic fiber surface. A multilayer of an enzyme, alkaline phosphatase, was successfully immobilized on an optical fiber surface. Chemiluminescence, ellipsometry, and surface plasmon resonance were used to characterize the structure and activity of the assembly. A chemiluminescence-based fiber optic biosensor utilizing this immobilization technique has been developed for the detection of organophosphorous-based pesticides. Detection of pesticide at sub-ppm level has been achieved for paraoxon.

Paper Details

Date Published: 24 April 1996
PDF: 9 pages
Proc. SPIE 2676, Biomedical Sensing, Imaging, and Tracking Technologies I, (24 April 1996); doi: 10.1117/12.238794
Show Author Affiliations
Zhongping Chen, U.S. Army Natick RD&E Ctr. (United States)
Harry Hong Gao, Univ. of Massachusetts/Lowell (United States)
Jayant Kumar, Univ. of Massachusetts/Lowell (United States)
Kenneth A. Marx, Univ. of Massachusetts/Lowell (United States)
Sukant K. Tripathy, Univ. of Massachusetts/Lowell (United States)
David L. Kaplan, U.S. Army Natick RD&E Ctr. (United States)

Published in SPIE Proceedings Vol. 2676:
Biomedical Sensing, Imaging, and Tracking Technologies I
Robert A. Lieberman; Halina Podbielska M.D.; Tuan Vo-Dinh, Editor(s)

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