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Proceedings Paper

Effect of Nd:YAG collagen synthesis by human pulp fibroblasts
Author(s): Rahmat A. Barkhordar; Qaiser P. Ghani; Belma A. Enriquez; M. Zamir Hussain
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Paper Abstract

The effect of Nd:YAG laser on tissue is dependant on treatment power, times and frequencies. In order to determine the biologic effect Nd:YAG laser radiation on dental pulp tissue, human dental pulp fibroblasts were obtained from extracted human teeth, and grown in DME containing 10% FBS and used as passage 6 - 7. Confluent cells were exposed to defocused laser energy (1 W, 10 Hz) using a pulsed (120 microsecond(s) ) fiberoptic delivered (320 micrometers fiber) Nd:YAG for 1 - 5 minutes. The cells were labeled with 10 (mu) Ci/ml of 3H proline for 16 hours. Untreated cultures were used as controls. Collagen synthesis was expressed as DPM X 10-3 mg/protein. The purpose of these measurements was to ascertain whether these radiation levels may induced a reparative response resulting in enhanced collagen synthesis. The results showed that at 1 - 5 minutes there was not significant effect. The tested lasing doses apparently did not cause any injurious effect to the fibroblasts, hence no increase in collagen synthesis.

Paper Details

Date Published: 23 April 1996
PDF: 4 pages
Proc. SPIE 2672, Lasers in Dentistry II, (23 April 1996); doi: 10.1117/12.238751
Show Author Affiliations
Rahmat A. Barkhordar, Univ. of California/San Francisco (United States)
Qaiser P. Ghani, Univ. of California/San Francisco (United States)
Belma A. Enriquez, Univ. of California/San Francisco (United States)
M. Zamir Hussain, Univ. of California/San Francisco (United States)


Published in SPIE Proceedings Vol. 2672:
Lasers in Dentistry II
Harvey A. Wigdor; John D. B. Featherstone; Joel M. White; Joseph Neev, Editor(s)

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