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Proceedings Paper

Rapid protein characterization using bioreactive mass spectrometer probe tips
Author(s): Randall W. Nelson; J. Kathleen Lewis; David Dogruel; Jennifer R. Krone; Peter W. Williams
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Paper Abstract

Methods have been developed for the rapid and sensitive mass spectrometric characterization of peptides. The approach uses bioreactive mass spectrometer probe tips which are capable of modifying proteins for analytical purpose. In the demonstrated case, enzymatic proteolysis is initiated upon application of analyte to the probe tips, time allowed for digestion, and the results analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The probe tips have been used for proteolytic mapping and partial sequence determination of picomol quantities of peptide while maintaining analysis times of approximately 5 minutes.

Paper Details

Date Published: 1 April 1996
PDF: 7 pages
Proc. SPIE 2680, Ultrasensitive Biochemical Diagnostics, (1 April 1996); doi: 10.1117/12.237628
Show Author Affiliations
Randall W. Nelson, Arizona State Univ. (United States)
J. Kathleen Lewis, Arizona State Univ. (United States)
David Dogruel, Arizona State Univ. (United States)
Jennifer R. Krone, Arizona State Univ. (United States)
Peter W. Williams, Arizona State Univ. (United States)


Published in SPIE Proceedings Vol. 2680:
Ultrasensitive Biochemical Diagnostics
Gerald E. Cohn; Steven A. Soper; C. H. Winston Chen, Editor(s)

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