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Proceedings Paper

Quantitative PCR by real-time detection
Author(s): Timothy M. Woudenberg; J. Stevens
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Paper Abstract

Using homogeneous 5' nuclease assay (an energy transfer probe) as the reporting system, the progress of the polymerase chain reaction is monitored by laser induced fluorescence. Progress from cycle to cycle and within the extension plateau of a given cycle is measured in the exponential, linear and plateau phases. Quantitation of initial concentration is best done using exponential phase data. Information on the dynamics of PCR and enzyme kinetics are an important byproduct of the investigation.

Paper Details

Date Published: 1 April 1996
PDF: 10 pages
Proc. SPIE 2680, Ultrasensitive Biochemical Diagnostics, (1 April 1996); doi: 10.1117/12.237619
Show Author Affiliations
Timothy M. Woudenberg, Perkin-Elmer Corp. (United States)
J. Stevens, Perkin-Elmer Corp. (United States)


Published in SPIE Proceedings Vol. 2680:
Ultrasensitive Biochemical Diagnostics
Gerald E. Cohn; Steven A. Soper; C. H. Winston Chen, Editor(s)

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