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Proceedings Paper

Design of separation media for DNA sequencing in capillaries
Author(s): Steve Menchen; Ben A. Johnson; Ramki Madabhushi; Mitchell A. Winnik
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Paper Abstract

Practical DNA sequencing in a capillary format requires sieving fluids that can be replaced after each sequencing experiment. Uncrosslinked water soluble polymers were used to prepare DNA sieving networks, both through simple entanglements of linear polymers, and by end- capping with hydrophobic tails; aqueous solutions of the end-capped polymers yield viscous networks held together by micellar crosslinks. Both networks are effective resolving media for DNA sequencing ladders, and flow under stress, which has enabled the design of an automated capillary DNA sequencing system composed of a gel pump interfaced to an electrophoresis apparatus. The sieving properties of these polymers are described.

Paper Details

Date Published: 1 April 1996
PDF: 10 pages
Proc. SPIE 2680, Ultrasensitive Biochemical Diagnostics, (1 April 1996); doi: 10.1117/12.237618
Show Author Affiliations
Steve Menchen, Perkin-Elmer Corp. (United States)
Ben A. Johnson, Perkin-Elmer Corp. (United States)
Ramki Madabhushi, Perkin-Elmer Corp. (United States)
Mitchell A. Winnik, Univ. of Toronto (Canada)


Published in SPIE Proceedings Vol. 2680:
Ultrasensitive Biochemical Diagnostics
Gerald E. Cohn; Steven A. Soper; C. H. Winston Chen, Editor(s)

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