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Proceedings Paper

Development of a bacteriophage displayed peptide library and biosensor
Author(s): Robert C. Chin; Noe Salazar; Michael W. Mayo; Victor Ivan Villavicencio; Richard B. Taylor; James P. Chambers; James J. Valdes
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Paper Abstract

A miniaturized, handheld biosensor for identification of hazardous biowarfare agents with high specificity is being developed. An innovative biological recognition system based on bacteriophage displayed peptide receptors will be utilized in conjunction with the miniature biosensor technology being developed. A bacteriophage library has been constructed to provide the artificial receptors. The library can contain millions of bacteriophage with randomly displayed peptide sequences in the phage outer protein coat which act as binding sites for the agents of interest. This library will be used to 'bio-pan' for phages that bind to a number of toxins and infectious agents and can, thus, provide an endless supply of low cost, reliable, specific, and stable artificial receptors. The biosensor instrument will utilize evanescent wave, planar waveguide, far-red dyes, diode laser and miniature circuit technologies for performance and portability.

Paper Details

Date Published: 1 April 1996
PDF: 11 pages
Proc. SPIE 2680, Ultrasensitive Biochemical Diagnostics, (1 April 1996); doi: 10.1117/12.237607
Show Author Affiliations
Robert C. Chin, Systems and Processes Engineering Corp. (United States)
Noe Salazar, Systems and Processes Engineering Corp. (United States)
Michael W. Mayo, Systems and Processes Engineering Corp. (United States)
Victor Ivan Villavicencio, Systems and Processes Engineering Corp. (United States)
Richard B. Taylor, Univ. of Texas/San Antonio (United States)
James P. Chambers, Univ. of Texas/San Antonio (United States)
James J. Valdes, U.S. Army Edgewood Research, Development and Engineering Ctr. (United States)

Published in SPIE Proceedings Vol. 2680:
Ultrasensitive Biochemical Diagnostics
Gerald E. Cohn; Steven A. Soper; C. H. Winston Chen, Editor(s)

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