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Proceedings Paper

DNA sequencing using four-color capillary array electrophoresis and energy-transfer primers
Author(s): Indu Kheterpal; Jingyue Ju; Arun Radhakrishnan; Gabriel S. Brandt; Charles L. Ginther; Steven M. Clark; James J. Scherer; George F. Sensabaugh; Richard A. Mathies
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Paper Abstract

A practical capillary array electrophoresis sequencer has been constructed and tested for large scale DNA sequencing. This system employs a scanning confocal optical system to detect DNA fragments electrophoresed on a capillary array. By using the recently developed energy transfer fluorescent primers, sensitive four-color detection is facilitated while exciting with just a single laser wavelength (488 nm) from an argon ion laser. Software for facile reduction of the images to four-color trace files and for automated base-calling have also been developed. This system can detect up to 25 capillaries at a time and has a raw sequencing rate of approximately 6 kilobases/hour. Applications to mitochondrial D-loop DNA sequencing are presented as a demonstration.

Paper Details

Date Published: 1 April 1996
PDF: 10 pages
Proc. SPIE 2680, Ultrasensitive Biochemical Diagnostics, (1 April 1996); doi: 10.1117/12.237606
Show Author Affiliations
Indu Kheterpal, Univ. of California/Berkeley (United States)
Jingyue Ju, Univ. of California/Berkeley (United States)
Arun Radhakrishnan, Univ. of California/Berkeley (United States)
Gabriel S. Brandt, Univ. of California/Berkeley (United States)
Charles L. Ginther, Univ. of California/Berkeley (United States)
Steven M. Clark, Univ. of California/Berkeley (United States)
James J. Scherer, Univ. of California/Berkeley (United States)
George F. Sensabaugh, Univ. of California/Berkeley (United States)
Richard A. Mathies, Univ. of California/Berkeley (United States)


Published in SPIE Proceedings Vol. 2680:
Ultrasensitive Biochemical Diagnostics
Gerald E. Cohn; Steven A. Soper; C. H. Winston Chen, Editor(s)

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