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Proceedings Paper

Autofluorescence spectroscopy of normal and malignant tissues: both in-vivo and ex-vivo measurements in the upper aero-digestive tract and lung tissues
Author(s): Ousama M. A'Amar; Dominique Lignon; O. Menard; Henri Begorre; Francois H. Guillemin; Edouard Yvroud
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Paper Abstract

A spectroscopic system with flexible three optical fiber sensor had been developed to study tissue fluorescence for a clinical use. Autofluorescence spectra at 413 nm and 10 mW excitation light power from different tissues in oral cavity had been measured in vivo in 25 subjects. The correlation coefficient in spectral shape between individual spectra and the mean emission spectrum of each site was about 0.9 and fluorescence intensity variation ranged between 20% and 45% according to the examined site. The variation in fluorescence intensity of the main emission wavelength at about 520 nm between spectra of the lower part of tongue, gingiva, lips, floor of cavity, cheek and palate was not statistically significant. But the spectrum of the upper part of tongue had been characterized by an additional peak around 635 nm. Otherwise, autofluorescence spectra at 410 nm and 0.5 mW excitation light power of 8 carcinoma of buccal and lung tissues were measured. The fluorescence ratio at 520 emission peak between normal tissue and carcinoma was evaluated at a maximum value of 13 for a lung cancer (ex vivo measurement) and a minimum of 3.3 for a cancer of the oro-pharynx (in vivo measurement). On the other hand, a fluorescence peak at 635 nm had characterized the carcinoma of the floor of cavity and the upper part of tongue.

Paper Details

Date Published: 5 April 1996
PDF: 9 pages
Proc. SPIE 2679, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy, (5 April 1996); doi: 10.1117/12.237596
Show Author Affiliations
Ousama M. A'Amar, IMAC-CRAN and CNRS (United States)
Dominique Lignon, IMAC-CRAN and CNRS (France)
O. Menard, CHU de Brabois (France)
Henri Begorre, CNRS (France)
Francois H. Guillemin, IMAC-CRAN and CNRS (France)
Edouard Yvroud, IMAC-CRAN and CNRS (France)


Published in SPIE Proceedings Vol. 2679:
Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy
Robert R. Alfano; Abraham Katzir, Editor(s)

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