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Proceedings Paper

Spatial distribution of tissue fluorescence
Author(s): Ivan S. Melnik; Sergiy M. Dets; Nikolay A. Denisov; Alexander M. Ovcharuk; Alexander Y. Joffe
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Paper Abstract

Fluorescence signal during tissue LIF-analysis depends on both excitation conditions and tissue optics, registration optics and location of the probe relative to tissue sample as well. To develop reliable fiber optic probes and optimize their position the spatial distribution of tissue fluorescence should be studied. Fluorescence indicatrices of skin of rat were measured in angular range of 80 degrees. Excited light from He-Cd (20 mW, 442 nm) laser was delivered on the cutaneous surface at the angular range from 0 to 60 degrees with the tissue surface. Fluorescence was registered in the spectrum between 530 nm and 700 nm with 1.5 nm resolution. Autofluorescence of the skin of 5 white rats was studied in-vivo. Local application of sensitizer hypericin was used for stimulated fluorescence studies. Fluorescence indicatrices were not corresponded to scattering ones under the same conditions and depended on incident angle of excitative laser beam. No influence of polarization of excitative beam on outside fluorescence distribution was observed. Maximum in-vivo fluorescence yield was registered at the normal incidence. There were observed marked differences between spatial distribution of normal and photosensitized rat skin tissues.

Paper Details

Date Published: 5 April 1996
PDF: 5 pages
Proc. SPIE 2679, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy, (5 April 1996); doi: 10.1117/12.237586
Show Author Affiliations
Ivan S. Melnik, National Technical Univ. of Ukraine (Canada)
Sergiy M. Dets, National Technical Univ. of Ukraine (Canada)
Nikolay A. Denisov, National Technical Univ. of Ukraine (Ukraine)
Alexander M. Ovcharuk, National Technical Univ. of Ukraine (Ukraine)
Alexander Y. Joffe, Ukranian Institute of Clinical and Experimental Surgery (Ukraine)

Published in SPIE Proceedings Vol. 2679:
Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy
Robert R. Alfano; Abraham Katzir, Editor(s)

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