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Proceedings Paper

Micro-optical properties of tissue
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Paper Abstract

The aim of this study was to develop quantitative methods for relating the microstructure of a tissue to the magnitude and wavelength dependence of its scattering coefficient. Two methods, cell counting and spatial frequency analysis, were used to estimate the distribution of sizes of structures imaged by light and electron microscopy. We found that scatterers in the epidermal layer of the skin exhibit a log-normal size distribution, whereas the spatial fluctuations in the index of refraction of dense fibrous tissues, such as the dermis, follow a power law. The correlations in the refractive indices of a variety of tissues exhibit characteristics of a random fractal with a Hurst coefficient between 0.3 and 0.5. Calculated from the measured distributions and volume fractions, the magnitudes of the scattering coefficient and anisotropy parameters of the tissue were found to be within the range 10 less than (mu) s less than 35 mm-1 and 0.7 less than g less than 0.97, depending on wavelength and tissue structure. Our results suggest that analysis of histological images of tissues is a viable method for estimating the optical parameters of tissues and their wavelength dependence.

Paper Details

Date Published: 5 April 1996
PDF: 11 pages
Proc. SPIE 2679, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy, (5 April 1996); doi: 10.1117/12.237578
Show Author Affiliations
Gitesh Kumar, Hong Kong Univ. of Science and Technology (United States)
Joseph M. Schmitt, Hong Kong Univ. of Science and Technology (United States)


Published in SPIE Proceedings Vol. 2679:
Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy
Robert R. Alfano; Abraham Katzir, Editor(s)

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