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Proceedings Paper

Cykotine mRNA expression in mouse retina after laser injury by reverse transcriptase-polymerase chain reaction (RT-PCR)
Author(s): Steven T. Schuschereba; Phillip D. Bowman; Veronica Ujimore; Stephen W. Hoxie; Jose M. Pizarro; Michael E. Cross; David J. Lund
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Paper Abstract

The purpose of this study was to identify cytokines produced by the retina after laser injury. With the aid of a scanning laser ophthalmoscope (SLO), right eyes of mice received lesions from a continuous wave argon laser. Left eyes served as unirradiated controls. At 2, 4, 6, 12, 24, and 48 hr after laser irradiation groups of 3 mice were euthanized and retinas fixed for histology or isolated for RNA. Messenger RNA (mRNA) was reverse-transcribed into complementary DNA (cDNA) and subjected to polymerase chain reaction for the following cytokines: tumor necrosis factor-(alpha) (TNF-(alpha) ), interleukin-1(alpha) /(Beta) (IL- 1(alpha) /(Beta) ), interleukin-6 (IL-6), transforming growth factor-(Beta) 1 (TGF- (Beta) 1), macrophage colony stimulating factor (M-CSF), inducible nitric oxide synthase (iNOS), and glyceraldehyde 3-phosphate dehydrogenase (G3PDH). Histologically, lesions were confined to the photoreceptors, retinal pigment epithelium, and choroid. In laser-injured retinas, mRNA levels were elevated for IL-1(alpha) , TGF-(Beta) 1, iNOS, and G3PDH, but not TNF-(alpha) , IL-1(Beta) , or IL-6. It appears that the retina, in response to laser injury, upregulates a select number of cytokines in a time-course dependent fashion.

Paper Details

Date Published: 1 April 1996
PDF: 11 pages
Proc. SPIE 2674, Laser-Inflicted Eye Injuries: Epidemiology, Prevention, and Treatment, (1 April 1996); doi: 10.1117/12.237503
Show Author Affiliations
Steven T. Schuschereba, Walter Reed Army Institute of Research (United States)
Phillip D. Bowman, U.S. Army Institute of Surgical Research (United States)
Veronica Ujimore, Walter Reed Army Institute of Research (United States)
Stephen W. Hoxie, Walter Reed Army Institute of Research (United States)
Jose M. Pizarro, Walter Reed Army Institute of Research (United States)
Michael E. Cross, Walter Reed Army Institute of Research (United States)
David J. Lund, Walter Reed Army Institute of Research (United States)


Published in SPIE Proceedings Vol. 2674:
Laser-Inflicted Eye Injuries: Epidemiology, Prevention, and Treatment
Bruce E. Stuck; Michael Belkin, Editor(s)

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