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Proceedings Paper

Application of avalanche photodiodes to confocal and confocal florescence microscopy
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Paper Abstract

We describe the use of avalanche photodiodes as solid state photon counters in scanning confocal microscopy. Photon counters have been of limited use for moderate-to-rapid image acquisition speeds due to comparatively low saturation count rates. Several approaches offer the promise of real-time photon-limited image acquisition using off-the-shelf components and dark count rates which are fully adequate for imaging. We characterize in some detail a gated NPN switch/pulse-bias circuit which offers excellent, economical performance over a wide wavelength range, and discuss specific imaging applications.

Paper Details

Date Published: 10 April 1996
PDF: 11 pages
Proc. SPIE 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III, (10 April 1996); doi: 10.1117/12.237495
Show Author Affiliations
Thomas G. Brown, The Institute of Optics/Univ. of Rochester (United States)
Stephen T. Kreger, The Institute of Optics/Univ. of Rochester (United States)


Published in SPIE Proceedings Vol. 2655:
Three-Dimensional Microscopy: Image Acquisition and Processing III
Carol J. Cogswell; Gordon S. Kino; Tony Wilson, Editor(s)

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