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Proceedings Paper

New technique for real-time testing and alignment of high-NA lenses
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Paper Abstract

A novel technique that provides a functional response, similar to the point spread function, for the characterization of high NA lenses in real time is presented. This measurement technique employs absorption and subsequent fluorescence in a bulk solution of a suitable fluorophore. Both the dye and the solvent can be chosen to match the experimental conditions best for which the lens is tested, especially with respect to the refractive index of the solvent and the wavelengths of excitation and emission. The experimental results presented show the sensitivity of the new technique to different apodization conditions of the lens. Theoretically the experiments are modeled from the Rayleigh-Sommerfeld integral of scalar diffraction theory in the Kirchhoff approximation. Applying the shift-invariance approximation to save computing time, theoretical curves are calculated without any fitting parameters that show excellent agreement with the experimental results.

Paper Details

Date Published: 10 April 1996
PDF: 10 pages
Proc. SPIE 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III, (10 April 1996); doi: 10.1117/12.237493
Show Author Affiliations
Michiel Mueller, Univ. of Amsterdam (Netherlands)
G. J. Brakenhoff, Univ. of Amsterdam (Netherlands)

Published in SPIE Proceedings Vol. 2655:
Three-Dimensional Microscopy: Image Acquisition and Processing III
Carol J. Cogswell; Gordon S. Kino; Tony Wilson, Editor(s)

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