Share Email Print

Proceedings Paper

Vectorial theory of confocal fluorescence
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Imaging in fluorescence microscopy is analyzed using a vectorial diffraction theory. Both conventional and confocal microscopy are considered. A fluorescent molecule is modeled as a radiating electric dipole. Two particular limiting cases are considered: the dipole can either freely rotate in space between excitation and emission, or is fixed in space. For each case, we average over all dipole orientations.

Paper Details

Date Published: 10 April 1996
PDF: 7 pages
Proc. SPIE 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III, (10 April 1996); doi: 10.1117/12.237488
Show Author Affiliations
Colin J. R. Sheppard, Univ. of Sydney (Australia)
Peter Torok, Univ. of Oxford (United Kingdom)

Published in SPIE Proceedings Vol. 2655:
Three-Dimensional Microscopy: Image Acquisition and Processing III
Carol J. Cogswell; Gordon S. Kino; Tony Wilson, Editor(s)

© SPIE. Terms of Use
Back to Top