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Proceedings Paper

Confocal imaging through a dielectric interface: aberration modeling
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Paper Abstract

The aberrations introduced when focusing within a specimen with a refractive index equal to that of water using an oil-immersion objective are investigated. The peak intensity in the confocal point spread function drops by a factor of two for focusing less than 10 micrometers into the specimen. The effects of scaling of dimensions in the resulting images are discussed. The image exhibits an axial stretching by a factor of about 1.12.

Paper Details

Date Published: 10 April 1996
PDF: 7 pages
Proc. SPIE 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III, (10 April 1996); doi: 10.1117/12.237486
Show Author Affiliations
Colin J. R. Sheppard, Univ. of Sydney (Australia)
Peter Torok, Univ. of Cambridge (United Kingdom)


Published in SPIE Proceedings Vol. 2655:
Three-Dimensional Microscopy: Image Acquisition and Processing III
Carol J. Cogswell; Gordon S. Kino; Tony Wilson, Editor(s)

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