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Proceedings Paper

Volumetric Raman spectral imaging with a confocal Raman microscope: image modalities and applications
Author(s): Colin J. H. Brenan; Ian Warwick Hunter; Michael J. Korenberg
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Paper Abstract

The Fourier transform Confocal Raman Microscope (FT-CRM) enables non-invasive 3D Raman spectroscopic analysis and visualization of chemically heterogeneous preparations. The instrument combines a confocal optical microscope with a visible light Fourier transform Raman spectrometer to acquire and analyze the Raman spectrum of light scattered from a voxel in the sample defined by the confocal optics. Scanning the sample relative to the confocal voxel and recording the Raman spectrum at each scan position generates a multi- dimensional data set encoding the spatially-varying compositional properties of the sample. We report here on the spatial and spectral FT-CRM image properties that includes recent work on correlation-based Raman spectroscopic imaging and application of parametric spectral estimators for robust Raman spectrum estimation.

Paper Details

Date Published: 10 April 1996
PDF: 10 pages
Proc. SPIE 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III, (10 April 1996); doi: 10.1117/12.237470
Show Author Affiliations
Colin J. H. Brenan, Massachusetts Institute of Technology (United States)
Ian Warwick Hunter, Massachusetts Institute of Technology (United States)
Michael J. Korenberg, Queen's Univ. (Canada)


Published in SPIE Proceedings Vol. 2655:
Three-Dimensional Microscopy: Image Acquisition and Processing III
Carol J. Cogswell; Gordon S. Kino; Tony Wilson, Editor(s)

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