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Proceedings Paper

Three-dimensional computed tomography using a double-axis fluorescence microscope
Author(s): Susumu Kikuchi; Kazuo Sonobe; Hidetoshi Yamada; Nagaaki Ohyama
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Paper Abstract

We developed a double-axis fluorescence microscope as a new type of 3D microscope, in which two conventional microscopes are placed perpendicularly to one another. This optical microscope provides a solution to extend the spatial frequency band in the depth direction, which is greatly limited for conventional single-axis microscopes. In addition, this equipment has a capability of implementing a fast image acquisition system by simultaneously taking images through both microscopes. We evaluated the 3D imaging properties of our system using 3D observation images of a micro-fluorescent sphere. In order to reconstruct 3D images of micro-objects, we applied a CT-based method we proposed to the 3D microscopic imaging system. Our experiments using a biological micro-specimen verified usefulness and effectiveness of our system.

Paper Details

Date Published: 10 April 1996
PDF: 7 pages
Proc. SPIE 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III, (10 April 1996); doi: 10.1117/12.237468
Show Author Affiliations
Susumu Kikuchi, Olympus Optical Co., Ltd. (Japan)
Kazuo Sonobe, Olympus Optical Co., Ltd. (Japan)
Hidetoshi Yamada, Olympus Optical Co., Ltd. (Japan)
Nagaaki Ohyama, Tokyo Institute of Technology (Japan)


Published in SPIE Proceedings Vol. 2655:
Three-Dimensional Microscopy: Image Acquisition and Processing III
Carol J. Cogswell; Gordon S. Kino; Tony Wilson, Editor(s)

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