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Proceedings Paper

Integrated optic sensor for measuring aflatoxin-B1 in corn
Author(s): Anthony A. Boiarski; James R. Busch; R. S. Brody; Richard W. Ridgway; Wolf P. Altman; C. Golden
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Paper Abstract

An integrated optic refractometer device was developed to perform a rapid one-step, homogeneous immunoassay. The device measures refractive index changes at the surface of a planar, singlemode, ion-exchange waveguide using difference interferometry. Anti-aflatoxin- B1 antibodies were attached to the waveguide surface to provide a bioselective coating for detecting and quantifying the aflatoxin-B1 antigen level in a sample. The detection limit of this small antigen must be determined using a competitive assay format. To determine feasibility of the competitive assay, we determined the biosensor response to a larger molecular weight competing antigen, namely HRP-labeled aflatoxin-B1. This labeled antigen will compete with unlabeled aflatoxin for binding sites on the sensor surface. Increased sample aflatoxin levels will result in a decreased time-dependent phase change of the helium-neon laser light beam. Phase change data were determined for various concentration levels of HRP-labeled aflatoxin- B1 antigen. The assay measurements were made over a 5-minute time period. Results indicated that a competitive assay is feasible. Future assay efforts should be able to demonstrate measurement of aflatoxin-B levels found in contaminated corn samples.

Paper Details

Date Published: 25 March 1996
PDF: 8 pages
Proc. SPIE 2686, Integrated Optics and Microstructures III, (25 March 1996); doi: 10.1117/12.236140
Show Author Affiliations
Anthony A. Boiarski, BioSense Consulting (United States)
James R. Busch, Battelle Memorial Institute/Columbus Div. (United States)
R. S. Brody, Battelle Memorial Institute/Columbus Div. (United States)
Richard W. Ridgway, Battelle Memorial Institute/Columbus Div. (United States)
Wolf P. Altman, Battelle Memorial Institute/Columbus Div. (United States)
C. Golden, Editek Inc. (United States)

Published in SPIE Proceedings Vol. 2686:
Integrated Optics and Microstructures III
Massood Tabib-Azar, Editor(s)

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