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Proceedings Paper

In situ off-axis holography of marine plankton
Author(s): Eric Paul Krantz; John Watson; Peter R. Hobson; R. S. Lampitt; A. Rogerson
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Paper Abstract

We present an off-axis transmission holographic technique for recording marine plankton in situ within a test tank of 36,000 ml with a pulsed laser in a 40 ns interval. The holographic plate is located in air and is therefore unaffected by aquatic conditions that may cause emulsion degradation (e.g. non-uniform swelling and surface contamination). The reference beam traverses a path in air only, and thus remains unaffected by dense concentrations of plankton. Third order aberrations, notably spherical aberration and astigmatism, are suppressed to yield an experimental resolution of 7 lp/mm (70 micrometers ) with a USAF 1951 target located 600 mm in water from the observation window. Plankton particle counts examined by real image reconstruction show a strong correlation with duplicate samples examined under a microscope.

Paper Details

Date Published: 25 March 1996
PDF: 8 pages
Proc. SPIE 2652, Practical Holography X, (25 March 1996); doi: 10.1117/12.236066
Show Author Affiliations
Eric Paul Krantz, Univ. of Aberdeen (United Kingdom)
John Watson, Univ. of Aberdeen (United Kingdom)
Peter R. Hobson, Brunel Univ. (United Kingdom)
R. S. Lampitt, Southampton Oceanography Ctr. (United Kingdom)
A. Rogerson, Univ. Marine Biological Station Millport (United Kingdom)

Published in SPIE Proceedings Vol. 2652:
Practical Holography X
Stephen A. Benton, Editor(s)

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