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Proceedings Paper

Gas-chromatography-tandem mass spectrometry (GC-MS) method for the determination of glycerol in human urine samples
Author(s): Manuela Dobrescu; Mirela Zorio; Mihai Ionica
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Paper Abstract

Glycerol is the key constituent to triglycerides and is an important compound participating in the lipid metabolism. It is an endogenous compound found in small amounts in human blood and urine. In high doses it can cause migraine, nausea, thirst1. The present degree has purpose the developing and validating a Gas-Chromatography- Tandem Mass Spectrometry (GC-MS) method accurate, rapid and easy to identify and quantify the glycerol analyte in urine samples. The applied analytical technique is based on derivation of the urine sample evaporated with MSTFA/NH4I/ethantiol derivatizing reagent. By this reaction, the hydroxyl functions of glycerol are converted to silyl derivatives, compounds having boiling points lower than the original compounds, this facilitating their GC-MS assay. For estimating the glycerol content, the method are monitored the mass fragments characteristic of the compound of interest – m/z 205, 218 and 293 and an ion corresponding to the deurated analog-m/z 2082.

Paper Details

Date Published: 31 December 2018
PDF: 6 pages
Proc. SPIE 10977, Advanced Topics in Optoelectronics, Microelectronics, and Nanotechnologies IX, 109773G (31 December 2018); doi: 10.1117/12.2324708
Show Author Affiliations
Manuela Dobrescu, Doping Control Lab. (Romania)
Mirela Zorio, Doping Control Lab. (Romania)
Mihai Ionica, Univ. Politehnica of Bucharest (Romania)
Ctr. for Military Medical Scientific Research (Romania)

Published in SPIE Proceedings Vol. 10977:
Advanced Topics in Optoelectronics, Microelectronics, and Nanotechnologies IX
Marian Vladescu; Razvan D. Tamas; Ionica Cristea, Editor(s)

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