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Proceedings Paper

Extending the performance of linear and nonlinear spatial frequency modulated imaging platforms (Conference Presentation)
Author(s): Jeffrey A. Squier; Nathan Worts; Alyssa Allende Motz; Jeff Field; Keith Wernsing; Patrick Stockton; Randy Bartels
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Paper Abstract

Through spatial frequency modulated imaging (SPIFI), multimodal, multiphoton microscopy (MPM) benefits from an extended excitation source without compromising the key performance characteristics afforded by point scanning MPM platforms. For example, the introduction of an in-house custom machined mask, which imparts a spatially distinct, temporal amplitude modulation to the extended excitation source, allows one and two-dimensional images to be captured with single element detection. This enables extended source imaging methods to retain a key feature of the point scanning systems; namely, the ability to image within scattering media, at depth. Further, the range of contrast mechanisms for the extended source techniques presented here are not limited and readily extend to both linear and nonlinear imaging modalities. The SPIFI method developed here enables facile detection of such images with the added benefit of enhanced resolution. Notably, the resolution improvement holds across contrast mechanisms, and is independent of whether the contrast is generated through linear or nonlinear processes. Significantly, phase also comes into play as we present new SPIFI geometries that illustrate the role of phase in strategically controlling the source geometry and/or generating image contrast.

Paper Details

Date Published: 17 September 2018
Proc. SPIE 10753, Ultrafast Nonlinear Imaging and Spectroscopy VI, 107530L (17 September 2018); doi: 10.1117/12.2321327
Show Author Affiliations
Jeffrey A. Squier, Colorado School of Mines (United States)
Nathan Worts, Colorado School of Mines (United States)
Alyssa Allende Motz, Colorado School of Mines (United States)
Jeff Field, Colorado State Univ. (United States)
Keith Wernsing, Colorado State Univ. (United States)
Patrick Stockton, Colorado State Univ. (United States)
Randy Bartels, Colorado State Univ. (United States)

Published in SPIE Proceedings Vol. 10753:
Ultrafast Nonlinear Imaging and Spectroscopy VI
Zhiwen Liu; Demetri Psaltis; Kebin Shi, Editor(s)

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