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Proceedings Paper

Probing membrane receptors with enhanced Raman imaging
Author(s): Lifu Xiao; Sian Sloan-Dennison; Zachary D. Schultz
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Paper Abstract

Our lab has shown that nanoparticles functionalized with short peptides can selectively bind to receptor proteins in vitro. Our results indicate that the Raman signals observed from purified receptors in surface enhanced Raman scattering (SERS) experiments match those observed with tip-enhanced Raman scattering (TERS) experiments performed on membrane receptors in intact cell membranes. Analysis of the observed Raman signals suggest the signals arise from the amino-acids in the protein receptor responsible for binding and recognition of the ligand attached to the nanoparticle probe. Further experiments show the variance in the data correlates with affinity of the nanoparticle probe with a specific receptor. This result illustrates a new approach to studying membrane receptors.

Paper Details

Date Published: 5 September 2018
PDF: 7 pages
Proc. SPIE 10726, Nanoimaging and Nanospectroscopy VI, 107260K (5 September 2018); doi: 10.1117/12.2321300
Show Author Affiliations
Lifu Xiao, The Ohio State Univ. (United States)
Sian Sloan-Dennison, The Ohio State Univ. (United States)
Zachary D. Schultz, The Ohio State Univ. (United States)

Published in SPIE Proceedings Vol. 10726:
Nanoimaging and Nanospectroscopy VI
Prabhat Verma; Alexander Egner, Editor(s)

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