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Proceedings Paper

Laser scanning confocal microscopy using illumination beams with different polarizations in quick succession
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Paper Abstract

The incorporation of polarization control on the illumination beam in a laser scanning confocal microscope allows extraction of the orientational information of submicroscopic features of a sample being studied. In this paper, we present the implementation of an optical arrangement that generates homogeneous as well as non-homogeneous user define polarization profiles over the cross-sectional area of a laser beam. A confocal system is built with this optical arrangement to obtain images of the same target with different polarized illumination beams such that there is considerable reduction in the time gap between two consecutive illuminations of each location of the sample.

Paper Details

Date Published: 18 September 2018
PDF: 9 pages
Proc. SPIE 10772, Unconventional and Indirect Imaging, Image Reconstruction, and Wavefront Sensing 2018, 107720I (18 September 2018); doi: 10.1117/12.2320176
Show Author Affiliations
Ranjan Kalita, Indian Institute of Technology Guwahati (India)
S. S. Goutam Buddha, Indian Institute of Technology Guwahati (India)
Bosanta R. Boruah, Indian Institute of Technology Guwahati (India)


Published in SPIE Proceedings Vol. 10772:
Unconventional and Indirect Imaging, Image Reconstruction, and Wavefront Sensing 2018
Jean J. Dolne; Philip J. Bones, Editor(s)

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