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Proceedings Paper

Multi-modal quantitative analysis of HeLa cells using digital holographic microscopy and confocal laser scanning microscopy
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Paper Abstract

In the paper the advantages of two different microscopic techniques, namely digital holographic microscopy (DHM) and Confocal Laser Scanning Microscopy (CLSM) have been combined with the aim to investigate HeLa cell culture in terms of statistical analysis of area of subcellular structures of HeLa cells and related to them dry mass estimation. To assure the proper statistical representation of the cells both measurements comprised of multiple fields of view (FoVs), stitched together to form two FoVs with overlapping regions. The results suggest a strong linear correlation of nucleoli dry mass to their projection area, a result that is promising in terms of its biological relevance.

Paper Details

Date Published: 7 September 2018
PDF: 7 pages
Proc. SPIE 10834, Speckle 2018: VII International Conference on Speckle Metrology, 108341V (7 September 2018); doi: 10.1117/12.2319588
Show Author Affiliations
Piotr Stępień, Warsaw Univ. of Technology (Poland)
Tytus Bernaś, Polish Academy of Science (Poland)
Wojciech Krauze, Warsaw Univ. of Technology (Poland)
Hanna Sas-Nowosielska, Polish Academy of Science (Poland)
Malgorzata Kujawińska, Warsaw Univ. of Technology (Poland)

Published in SPIE Proceedings Vol. 10834:
Speckle 2018: VII International Conference on Speckle Metrology
Malgorzata Kujawińska; Leszek R. Jaroszewicz, Editor(s)

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