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Incoherent holographic imaging of subsurface structures with volume holographic gratings
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Paper Abstract

Under broadband illumination, a multiplane microscopy incorporating volume holographic gratings (VHGs) to observe three-dimensional structures of biological samples with different depth simultaneously is presented. VHGs formed in thick recording materials, including PQ-PMMA, provide strong angular and wavelength transmittance filtering properties, which enable acquiring spatial–spectral images of fine structures within biological samples. Here, we experimentally demonstrate this microscopic imaging capability to obtain multiple depth-resolved mixed pollen grains images of fine structures from eight depths in one shot.

Paper Details

Date Published: 24 April 2018
PDF: 3 pages
Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 107111A (24 April 2018); doi: 10.1117/12.2319365
Show Author Affiliations
Yu-Hsin Chia, National Taiwan Univ. (Taiwan)
Hung-Chun Wang, National Tsing Hua Univ. (Taiwan)
Yuan Luo, National Taiwan Univ. (Taiwan)


Published in SPIE Proceedings Vol. 10711:
Biomedical Imaging and Sensing Conference
Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji; Yuan Luo, Editor(s)

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