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Optical trapping of quantum-dot conjugated AMPA-type receptors depend on initial assembling states
Author(s): Tatsunori Kishimoto; Yasuyo Maezawa; Suguru N. Kudoh; Takahisa Taguchi; Chie Hosokawa
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Paper Abstract

AMPA-type glutamate receptor (AMPAR) is one of the major neurotransmitter receptors at excitatory synapses. The initial assembling states of AMPARs at cell surface are essential for synaptic transmission, which is related with learning and memory in living neural systems. To realize artificial control of synaptic transmission, we demonstrate to modulate the initial assembling states of quantum-dot conjugated AMPARs (QD-AMPARs) with optical trapping. The optical trapping dynamics of QD-AMPARs on living neurons was evaluated with fluorescence imaging and fluorescence correlation spectroscopy (FCS). The transit time at laser focus of QD-AMPARs on neurons estimated from FCS analysis increased with the culturing days and addition of neurotransmitter, which suggests that QD-AMPARs are confined at the focal spot due to optical trapping.

Paper Details

Date Published: 24 April 2018
PDF: 2 pages
Proc. SPIE 10712, Optical Manipulation Conference, 1071204 (24 April 2018); doi: 10.1117/12.2319362
Show Author Affiliations
Tatsunori Kishimoto, National Institute of Advanced Industrial Science and Technology (Japan)
Kwansei Gakuin Univ. (Japan)
Yasuyo Maezawa, National Institute of Advanced Industrial Science and Technology (Japan)
Suguru N. Kudoh, Kwansei Gakuin Univ. (Japan)
Takahisa Taguchi, National Institute of Information and Communications Technology (Japan)
Chie Hosokawa, National Institute of Advanced Industrial Science and Technology (Japan)
Kwansei Gakuin Univ. (Japan)
AIST-Osaka Univ. (Japan)


Published in SPIE Proceedings Vol. 10712:
Optical Manipulation Conference
Takashige Omatsu; Hajime Ishihara; Keiji Sasaki, Editor(s)

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