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Analysis of collagen fiber orientation in biological tissues using polarization-resolved second-harmonic-generation microscopy
Author(s): Takuya Sakaue; Eiji Hase; Takeo Minamikawa; Takeshi Yasui
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Paper Abstract

Collagen plays an important role as a structural protein to determine the morphology and the mechanical property of tissues. Collagen orientation is one important parameter closely related with the mechanical property because collagen possesses fiber structure in tissues. However, it is difficult to evaluate the collagen fiber orientation quantitatively without invasiveness. In this article, we constructed continuously-polarization-resolved second-harmonic-generation (SHG) microscopy based on rapid polarization rotation every 15 degrees with electric-optic modulator, and applied it for the quantitative analysis of collagen fiber orientation. The orientation angle and degree of collagen fiber in different biological tissues were extracted from the curve fitting with a model function to a series of polarization-resolved

Paper Details

Date Published: 24 April 2018
PDF: 3 pages
Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 107111D (24 April 2018); doi: 10.1117/12.2319273
Show Author Affiliations
Takuya Sakaue, Tokushima Univ. (Japan)
Eiji Hase, Tokushima Univ. (Japan)
Takeo Minamikawa, Tokushima Univ. (Japan)
Takeshi Yasui, Tokushima Univ. (Japan)

Published in SPIE Proceedings Vol. 10711:
Biomedical Imaging and Sensing Conference
Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji; Yuan Luo, Editor(s)

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