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Hyperspectral digital holographic microscopy approach for reduction of coherence induced disturbances in quantitative phase imaging of biological specimens
Author(s): Álvaro Barroso; Steffi Ketelhut; Peter Heiduschka; Lena Kastl; Jürgen Schnekenburger; Björn Kemper
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Paper Abstract

Coherence induced noise and parasitic reflections in the experimental setup are main restrictions that limit the resolution and measurement accuracy in laser light-based digital holographic microscopy (DHM). We explored, if coherence properties of partial coherent light sources can be mimicked by utilizing spectrally tunable lasers. Moreover, the performance for label-free quantitative phase imaging of biological specimens is illustrated utilizing an experimental configuration including a commercial microscope and tunable super continuum laser sources with a wavelength range of up to 230 nm.

Paper Details

Date Published: 7 September 2018
PDF: 6 pages
Proc. SPIE 10834, Speckle 2018: VII International Conference on Speckle Metrology, 108340I (7 September 2018); doi: 10.1117/12.2319179
Show Author Affiliations
Álvaro Barroso, Univ. Münster (Germany)
Steffi Ketelhut, Univ. Münster (Germany)
Peter Heiduschka, Univ. Münster Medical Ctr. (Germany)
Lena Kastl, Univ. Münster (Germany)
Jürgen Schnekenburger, Univ. Münster (Germany)
Björn Kemper, Univ. Münster (Germany)

Published in SPIE Proceedings Vol. 10834:
Speckle 2018: VII International Conference on Speckle Metrology
Malgorzata Kujawińska; Leszek R. Jaroszewicz, Editor(s)

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