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Speckle imaging for monitoring the growth kinetics of Bacillus thuringiensis
Author(s): Hadi Loutfi; Hassan Badawi; Fabrice Pellen; Bernard Le Jeune; Roger Lteif; Mireille Kallassy; Guy Le Brun; Marie Abboud
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Paper Abstract

This paper presents an application of laser speckle imaging method to characterize the kinetic growth of Bacillus thuringiensis (Bt). Numbers of parameters, such as speckle grain size and spatial contrast, are considered in order to characterize the culture medium and to monitor in real time the fermentation process. We show that the grain size and the contrast of the speckle image decrease with the increase of the cells concentration. The correlation of speckle results with optical density measurements shows the effectiveness of dynamic speckle for real-time monitoring of Bt cells growth kinetics.

Paper Details

Date Published: 7 September 2018
PDF: 5 pages
Proc. SPIE 10834, Speckle 2018: VII International Conference on Speckle Metrology, 108341C (7 September 2018); doi: 10.1117/12.2318678
Show Author Affiliations
Hadi Loutfi, Saint Joseph Univ. (Lebanon)
Lab. OPTIMAG, IBSAM, Univ. de Bretagne Occidentale (France)
Hassan Badawi, Lab. OPTIMAG, IBSAM, Univ. de Bretagne Occidentale (France)
Fabrice Pellen, Lab. OPTIMAG, IBSAM, Univ. de Bretagne Occidentale (France)
Bernard Le Jeune, Lab. OPTIMAG, IBSAM, Univ. de Bretagne Occidentale (France)
Roger Lteif, Saint Joseph Univ. (Lebanon)
Mireille Kallassy, Saint Joseph Univ. (Lebanon)
Guy Le Brun, Lab. OPTIMAG, IBSAM, Univ. de Bretagne Occidentale (France)
Marie Abboud, Saint Joseph Univ. (Lebanon)


Published in SPIE Proceedings Vol. 10834:
Speckle 2018: VII International Conference on Speckle Metrology
Malgorzata Kujawińska; Leszek R. Jaroszewicz, Editor(s)

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