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Application of scan-less two-dimensional confocal microscopy achieved by a combination of confocal slit with wavelength/space conversion
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Paper Abstract

Confocal laser microscope (CLM) has been widely used in the fields of the non-contact surface topography, biomedical imaging, and other applications, because the confocality gives two-dimensional (2D) optical-sectioning or threedimensional (3D) imaging capability and the stray light elimination. One potential method for scan-less 2D CLM is a combination of the line-focused CLM with the 1D spectral encoding CLM. In this paper, we constructed such scan-less 2D CLM with the image acquisition time of 0.23 ms, the lateral resolution of 1.2 μm, the depth resolution of 2.4 μm, and apply it for in situ imaging of plant leaf to investigate a potential for plant health monitoring.

Paper Details

Date Published: 24 April 2018
PDF: 5 pages
Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 107111O (24 April 2018); doi: 10.1117/12.2318671
Show Author Affiliations
Eiji Hase, Tokushima Univ. (Japan)
JST, ERATO MINOSHIMA Intelligent Optical Synthesizer (IOS) (Japan)
Takeo Minamikawa, Tokushima Univ. (Japan)
Shuji Miyamoto, Tokushima Univ. (Japan)
JST, ERATO MINOSHIMA Intelligent Optical Synthesizer (IOS) (Japan)
Yasuhiro Mizutani, JST, ERATO MINOSHIMA Intelligent Optical Synthesizer (IOS) (Japan)
Osaka Univ. (Japan)
Tetsuo Iwata, Tokushima Univ. (Japan)
JST, ERATO MINOSHIMA Intelligent Optical Synthesizer (IOS) (Japan)
Hirotsugu Yamamoto, JST, ERATO MINOSHIMA Intelligent Optical Synthesizer (IOS) (Japan)
Utsunomiya Univ. (Japan)
Takeshi Yasui, Tokushima Univ. (Japan)
JST, ERATO MINOSHIMA Intelligent Optical Synthesizer (IOS) (Japan)


Published in SPIE Proceedings Vol. 10711:
Biomedical Imaging and Sensing Conference
Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji; Yuan Luo, Editor(s)

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