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Proceedings Paper

Opto electronic tweezers based smart sweeper for cells/micro-particles sorting
Author(s): R. S. Verma; N. Kumar
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Paper Abstract

We report on use of opto-electronic tweezers based sorting approach, termed as smart sweepers, for sorting the microscopic particles by using the Dielectrophoretic (DEP) force response of cells on applied a.c. bias frequency. The applied a.c. bias was kept in negative DEP region, close to the crossover frequency of one of the particles. A line shaped intensity pattern, generated by a cylindrical lens, was scanned across the mixture sample. The particles whose cross over frequency was close to the applied bias frequency, experienced negligible negative DEP(n-DEP) force. On the other hand, the other type of particle experienced large repelling force and were forced to move along the scanning direction of the line shaped intensity profile. We, as a proof of concept, demonstrated the working principle of opto electronic smart sweepers by sweeping out the polystyrene particles from a mixture consisting of polystyrene microspheres (PSM) and red blood cells (RBCs) and leaving RBCs in the region of interest.

Paper Details

Date Published: 26 April 2018
PDF: 6 pages
Proc. SPIE 10716, Saratov Fall Meeting 2017: Optical Technologies in Biophysics and Medicine XIX, 1071613 (26 April 2018); doi: 10.1117/12.2317744
Show Author Affiliations
R. S. Verma, Raja Ramanna Ctr. for Advanced Technology (India)
Homi Bhabha National Institute (India)
N. Kumar, Raja Ramanna Ctr. for Advanced Technology (India)

Published in SPIE Proceedings Vol. 10716:
Saratov Fall Meeting 2017: Optical Technologies in Biophysics and Medicine XIX
Valery V. Tuchin; Elina A. Genina; Vladimir L. Derbov; Dmitry Engelevich Postnov, Editor(s)

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