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Proceedings Paper

Scan-less, line-filed, confocal phase imaging with dual-comb microscopy
Author(s): Eiji Hase; Takeo Minamikawa; Shuji Miyamoto; Yasuhiro Mizutani; Tetsuo Iwata; Hirotsugu Yamamoto; Takeshi Yasui
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Paper Abstract

We introduce an optical frequency comb (OFC) to microscopy to coherently link the confocal microscopy and phase microscopy. One-dimensional (1D) image pixels of a sample were encoded onto OFC modes via 1D spectral encoding, in which OFC acted as an optical carrier with a vast number of discrete frequency channels. Then, a scan-less line-field confocal image with a depth resolution of 50 μm was decoded from a mode-resolved OFC amplitude spectrum obtained by dual-comb spectroscopy. Furthermore, a phase image with a depth resolution of sub-λ was decoded from a moderesolved OFC phase spectrum under the above confocality. The proposed hybrid microscopy approach will be a powerful tool for a variety of applications.

Paper Details

Date Published: 24 April 2018
PDF: 4 pages
Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 1071120 (24 April 2018); doi: 10.1117/12.2317029
Show Author Affiliations
Eiji Hase, Tokushima Univ. (Japan)
JST, ERATO MINOSHIMA Intelligent Optical Synthesizer (IOS) (Japan)
Takeo Minamikawa, Tokushima Univ. (Japan)
JST, ERATO MINOSHIMA Intelligent Optical Synthesizer (IOS) (Japan)
Shuji Miyamoto, Tokushima Univ. (Japan)
JST, ERATO MINOSHIMA Intelligent Optical Synthesizer (IOS) (Japan)
Yasuhiro Mizutani, JST, ERATO MINOSHIMA Intelligent Optical Synthesizer (IOS) (Japan)
Osaka Univ. (Japan)
Tetsuo Iwata, Tokushima Univ. (Japan)
JST, ERATO MINOSHIMA Intelligent Optical Synthesizer (IOS) (Japan)
Hirotsugu Yamamoto, JST, ERATO MINOSHIMA Intelligent Optical Synthesizer (IOS) (Japan)
Utsunomiya Univ. (Japan)
Takeshi Yasui, Tokushima Univ. (Japan)
JST, ERATO MINOSHIMA Intelligent Optical Synthesizer (IOS) (Japan)


Published in SPIE Proceedings Vol. 10711:
Biomedical Imaging and Sensing Conference
Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji; Yuan Luo, Editor(s)

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