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Non-scanning in-vivo three-dimensional hybrid structured illumination microscopy
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Paper Abstract

Imaging a volumetric sample in real time is required to directly observe biological anatomy and mechanisms. There are a variety of microscopic imaging systems, developed to achieve optical sectioning, such as confocal microscopy. However, the drawbacks of existing systems are long scanning time or strong laser power. To overcome such problems and to obtain high-speed optical sectioning images, we demonstrate HiLo structured illumination microscopy by the use of digital micro-mirror device (DMD) and the focal tunable lens (FTL). The proposed system is configured such that hybrid uniform and non-uniform pattern are projected onto DMD to reconstruct optical sectioning images. In addition, FTL is utilized to digitally change observation plane with constant magnification and resolution; thus, mechanical movement is eliminated. Furthermore, in vivo three-dimensional (3D) images of biological samples, including a live Canenorhabditis elegans, are experimentally demonstrated.

Paper Details

Date Published: 24 April 2018
PDF: 3 pages
Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 107110L (24 April 2018); doi: 10.1117/12.2316752
Show Author Affiliations
Ju Hsuan Chien, National Taiwan Univ. (Taiwan)
Chen Yen Lin, National Taiwan Univ. (Taiwan)
Yuan Luo, National Taiwan Univ. (Taiwan)


Published in SPIE Proceedings Vol. 10711:
Biomedical Imaging and Sensing Conference
Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji; Yuan Luo, Editor(s)

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