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Motion-picture phase imaging by an integrated optical system of a parallel phase-shifting digital holographic microscope
Author(s): Kazuki Shimizu; Takahito Fukuda; Tatsuya Hirakawa; Peng Xia; Yasuhiro Awatsuji; Kenzo Nishio; Osamu Matoba
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Paper Abstract

A parallel phase-shifting digital holographic microscope achieves motion-picture phase imaging of a dynamic minute specimen. However, much time and skill are required to construct the optical system of the microscope. The authors designed and constructed an optical system of a parallel phase-shifting digital holographic microscope in which the optical components of the microscope were integrated on a breadboard standing perpendicular to an optical table. The lateral and longitudinal magnifications of the microscope are 10 and 100, respectively. The authors experimentally demonstrated the motion-picture phase imaging of a dynamic minute specimen by the microscope. The holograms were recorded at 1,000 fps and the shutter speed was 0.5 ms.

Paper Details

Date Published: 24 April 2018
PDF: 4 pages
Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 1071115 (24 April 2018); doi: 10.1117/12.2316616
Show Author Affiliations
Kazuki Shimizu, Kyoto Institute of Technology (Japan)
Takahito Fukuda, Kyoto Institute of Technology (Japan)
Tatsuya Hirakawa, Kyoto Institute of Technology (Japan)
Peng Xia, National Institute of Advanced Industrial Science and Technology (Japan)
Yasuhiro Awatsuji, Kyoto Institute of Technology (Japan)
Kenzo Nishio, Kyoto Institute of Technology (Japan)
Osamu Matoba, Kobe Univ. (Japan)


Published in SPIE Proceedings Vol. 10711:
Biomedical Imaging and Sensing Conference
Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji; Yuan Luo, Editor(s)

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