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Implementation of stimulated Raman losses and stimulated Raman gain microscopy using three femtosecond laser sources
Author(s): Rajeev Ranjan; Annalisa D'Arco; Maria Antonietta Ferrara; Maurizio Indolfi; Michele Larobina; Luigi Sirleto
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Paper Abstract

Stimulated Raman scattering microscopy allows vibrational contrast mechanism for imaging with high spectral and spatial resolution along with three-dimensional sectioning. In this paper, the implementation of a Stimulated Raman Scattering microscope (SRSM), obtained by the integration of a femtosecond SRS spectroscopy set-up with an optical microscope equipped with a scanning unit, is described. Femtosecond Stimulated Raman Scattering microscope is equipped with three femtosecond laser sources: a Ti:Sapphire (Ti:Sa), a synchronized optical parametric oscillator (SOPO) and a frequency converters for ultrafast lasers, i.e. a second harmonic generator optimized for the SOPO. The proposed implementation allows to cover all the regions of Raman spectra, taking advantage of two different laser combinations. The first, Ti:Sa and SHG laser combination can cover in SRL modality the fingerprint region (500 − 1700 cm−1 ) and the silent region. The second, Ti:Sa and OPO, can cover the C-H region or O−H region (2800 − 3200 cm−1 ) in SRG modality. In order to demonstrate its successful realization Stimulated Raman Gain (SRG) and Stimulated Raman Losses (SRL) images of polystyrene beads are reported and discussed.

Paper Details

Date Published: 14 May 2018
PDF: 8 pages
Proc. SPIE 10684, Nonlinear Optics and its Applications 2018, 106842J (14 May 2018); doi: 10.1117/12.2315967
Show Author Affiliations
Rajeev Ranjan, Istituto per la Microelettronica e Microsistemi (Italy)
Annalisa D'Arco, Istituto per la Microelettronica e Microsistemi (Italy)
Maria Antonietta Ferrara, Istituto per la Microelettronica e Microsistemi (Italy)
Maurizio Indolfi, Istituto per la Microelettronica e Microsistemi (Italy)
Michele Larobina, Institute of Biostructures and Biomimaging (Italy)
Luigi Sirleto, Istituto per la Microelettronica e Microsistemi (Italy)


Published in SPIE Proceedings Vol. 10684:
Nonlinear Optics and its Applications 2018
Benjamin J. Eggleton; Neil G. R. Broderick; Anna C. Peacock, Editor(s)

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