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Proceedings Paper

Uptake, subcellular localization, and phototoxicity of photosensitizing porphyrins
Author(s): Herbert Schneckenburger; Reinhard Sailer; Michael H. Gschwend; Karin Kunzi-Rapp; Nermin Akguen; Inna Tregub; Wolfgang S. L. Strauss
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Paper Abstract

Cytotoxicity, uptake, intracellular location and phototoxicity were compared for the naturally occurring porphyrins uroporphyrin III (UP), coproporphyrin III (CP) and protoporphyrin IX (PP), as well as for tetra-, tri-, di- and monosulphonated meso-tetraphenyl porphyrins (TPPSn with n equals 4, 3, 2a, 1) in cultivated endothelial cells from calf aorta (BKEz-7). In addition, the uptake of CP and PP in the chick chorioallantoic membrane (CAM) was studied after intravenous application. Hydrophilic porphyrins (UP, CP, TPPS4 and TPPS3) exhibit low cellular uptake as well as low cyto- and phototoxicity as compared with the more hydrophobic compounds. UP, CP, TPPS4 and TPPS3 were mainly located in lysosomes, whereas PP, TPPS2a and TPPS1 were preferentially observed in the cytoplasm -- supposed to be located in cellular membranes of organelles -- and exhibited pronounced light-induced reactions. A rapid uptake by the CAM vasculature (5 min. - 30 min. after application) and some delayed uptake by the CAM matrix (30 min. - 6 h with a maximum at 60 min.) were measured for CP and PP.

Paper Details

Date Published: 31 January 1996
PDF: 9 pages
Proc. SPIE 2625, Photochemotherapy: Photodynamic Therapy and Other Modalities, (31 January 1996); doi: 10.1117/12.230975
Show Author Affiliations
Herbert Schneckenburger, Univ. Ulm and Fachhochschule Aalen (Germany)
Reinhard Sailer, Univ. Ulm (Germany)
Michael H. Gschwend, Univ. Ulm (Germany)
Karin Kunzi-Rapp, Univ. Ulm (Germany)
Nermin Akguen, Univ. Ulm (Germany)
Inna Tregub, Univ. Ulm (Germany)
Wolfgang S. L. Strauss, Univ. Ulm (Germany)

Published in SPIE Proceedings Vol. 2625:
Photochemotherapy: Photodynamic Therapy and Other Modalities
Benjamin Ehrenberg; Giulio Jori; Johan Moan, Editor(s)

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