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Fast spatial domain reconstruction for structured illumination microscopy
Author(s): Xing Zhou; Ming Lei; Dan Dan; Baoli Yao
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Paper Abstract

As a wide-field super-resolution (SR) technique, structured illumination microscopy (SIM) features the merits of fast imaging speed, low excitation intensity and a large field of view. However, it is hard for SIM to realize real-time imaging as the SR-image restoration always time-consuming. The basic workflow for conventional SIM reconstruction is based on the frequency domain reconstruction (FDR), which inevitably brings some computational errors and slows down the image reconstruction speed. In this letter, we introduce a fast SIM reconstruction scheme named spatial domain reconstruction (SDR) algorithm, which allow for obtaining a much faster reconstruction than FDR, making it suitable for real-time SR imaging. Meanwhile, the validity of the SDR algorithm is verified by real experiment.

Paper Details

Date Published: 24 April 2018
PDF: 2 pages
Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 107110N (24 April 2018); doi: 10.1117/12.2309616
Show Author Affiliations
Xing Zhou, Xi’an Institute of Optics and Precision Mechanics (China)
Ming Lei, Xi’an Institute of Optics and Precision Mechanics (China)
Dan Dan, Xi’an Institute of Optics and Precision Mechanics (China)
Baoli Yao, Xi’an Institute of Optics and Precision Mechanics (China)

Published in SPIE Proceedings Vol. 10711:
Biomedical Imaging and Sensing Conference
Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji; Yuan Luo, Editor(s)

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