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Proceedings Paper

Measurement of wavefront aberrations in cortex and peripheral nerve using a two-photon excitation guidestar
Author(s): Gregory L. Futia; Arjun Fontaine; Connor McCullough; Baris N. Ozbay; Nickolas M. George; John Caldwell; Diego Restrepo; Richard Weir; Emily A. Gibson
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Paper Abstract

Neural-machine interfaces using optogenetics are of interest due to their minimal invasiveness and potential for parallel read in and read out of activity. One possible biological target for such an interface is the peripheral nerve, where axonlevel imaging or stimulation could greatly improve interfacing with artificial limbs or enable neuron/fascicle level neuromodulation in the vagus nerve. Two-photon imaging has been successful in imaging brain activity using genetically encoded calcium or voltage indicators, but in the peripheral nerve, this is severely limited by scattering and aberrations from myelin. We employ a Shack-Hartman wavefront sensor and two-photon excitation guidestar to quantify optical scattering and aberrations in peripheral nerves and cortex. The sciatic and vagus nerves, and cortex from a ChAT-Cre ChR-eYFP transgenic mouse were excised and imaged directly. In peripheral nerves, defocus was the strongest aberration followed by astigmatism and coma. Peripheral nerve had orders of magnitude higher aberration compared with cortex. These results point to the potential of adaptive optics for increasing the depth of two-photon access into peripheral nerves.

Paper Details

Date Published: 23 February 2018
PDF: 6 pages
Proc. SPIE 10502, Adaptive Optics and Wavefront Control for Biological Systems IV, 105020J (23 February 2018); doi: 10.1117/12.2309492
Show Author Affiliations
Gregory L. Futia, Univ. of Colorado Denver (United States)
Arjun Fontaine, Univ. of Colorado Denver (United States)
Connor McCullough, Univ. of Colorado Denver (United States)
Baris N. Ozbay, Univ. of Colorado Denver (United States)
Nickolas M. George, Univ. of Colorado Denver (United States)
John Caldwell, Univ. of Colorado Denver (United States)
Diego Restrepo, Univ. of Colorado Denver (United States)
Richard Weir, Univ. of Colorado Denver (United States)
Emily A. Gibson, Univ. of Colorado Denver (United States)


Published in SPIE Proceedings Vol. 10502:
Adaptive Optics and Wavefront Control for Biological Systems IV
Thomas G. Bifano; Joel Kubby; Sylvain Gigan, Editor(s)

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