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Proceedings Paper

Deep-tissue two-photon imaging in brain and peripheral nerve with a compact high-pulse energy ytterbium fiber laser
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Paper Abstract

Two-photon microscopy is a powerful tool of current scientific research, allowing optical visualization of structures below the surface of tissues. This is of particular value in neuroscience, where optically accessing regions within the brain is critical for the continued advancement in understanding of neural circuits. However, two-photon imaging at significant depths have typically used Ti:Sapphire based amplifiers that are prohibitively expensive and bulky. In this study, we demonstrate deep tissue two-photon imaging using a compact, inexpensive, turnkey operated Ytterbium fiber laser (Y-Fi, KM Labs). The laser is based on all-normal dispersion (ANDi) that provides short pulse durations and high pulse energies. Depth measurements obtained in ex vivo mouse cortex exceed those obtainable with standard two-photon microscopes using Ti:Sapphire lasers. In addition to demonstrating the capability of deep-tissue imaging in the brain, we investigated imaging depth in highly-scattering white matter with measurements in sciatic nerve showing limited optical penetration of heavily myelinated nerve tissue relative to grey matter.

Paper Details

Date Published: 13 February 2018
PDF: 6 pages
Proc. SPIE 10492, Optical Interactions with Tissue and Cells XXIX, 1049217 (13 February 2018); doi: 10.1117/12.2309490
Show Author Affiliations
Arjun K. Fontaine, Univ. of Colorado Denver (United States)
Matthew S. Kirchner, Kapteyn-Murnane Labs., Inc. (United States)
John H. Caldwell, Univ. of Colorado Denver (United States)
Richard F. Weir, Univ. of Colorado Denver (United States)
Emily A. Gibson, Univ. of Colorado Denver (United States)


Published in SPIE Proceedings Vol. 10492:
Optical Interactions with Tissue and Cells XXIX
E. Duco Jansen; Hope Thomas Beier, Editor(s)

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